Generator

Part:BBa_K4419026:Design

Designed by: Leonardo ken Okumura, Ryoko Enomoto   Group: iGEM22_TokyoTech   (2022-10-11)


pCAG-loxP_NLS_NCre_IRES_GFP_loxP_mCherry (Mosquitoes)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1018
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2674
    Illegal AgeI site found at 704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2639


Design Notes

Mosquito (Anopheles gambiae str. PEST) codon optimization on proteins N-Cre and mCherry was performed by using VectorBuilder.


Source

N/A

References